Test method for Centella Asiatica Extract Total Glucosides — (CP2010)

Centella Asiatica Extract Supplier thiab Gotu Kola Extract Chaw tsim tshuaj paus

【Kev piav qhia】Tsaus daj rau faint brownish-daj hmoov; tsw tsw, tsw tsw; saj, ntsim; me ntsis hygroscopic. Solublein dej thiab ethanol. Insoluble hauv chloroform thiab ether.

【Identification】

(1) Muab 2 mg rau hauv ib lub raj tshuaj, ntxiv 1 ml ntawm anhydride acetic, thiab co zoo. Maj mam ntxiv 1 ml ntawm sulfuric acid raws li phab ntsa ntawm lub raj, thiab lub nplhaib violet-liab yog tsim rau ntawm ob-kua ciam teb.

(2) Dissolve ib tug kom muaj nuj nqis nyob rau hauv ethanol los tsim ib tug tov uas muaj 10 mg ib ml raws li cov tshuaj ntsuam xyuas. Nyias nyias ib tug ntau ntawm madecassoside CRS thiab asiaticoside CRS, ntxiv ethanol los npaj cov kev daws teeb meem uas muaj 10 mg ib ml, feem, siv raws li cov kev daws teeb meem. Ua cov txheej txheem rau nyias txheej chromatography (Appendix Ⅳ B). Siv silica gel G raws li cov txheej txheem txheej thiab txheej txheej sab saud ntawm kev sib xyaw ntawm n-butanol, ethyl acetate thiab dej (4: 1: 5) raws li theem mobile. Siv cais 5 µl ntawm txhua qhov ntawm peb txoj kev daws rau lub phaj. Tom qab tsim thiab tshem tawm cov phaj, qhuav hauv huab cua. Txau nrog cov tshuaj uas muaj anhydride acetic, sulfuric acid, thiab anhydrous ethanol (1: 1: 10), cua sov ntawm 105 degree kom pom tseeb. Ob qhov xim xiav-xim av nyob rau hauv chromatogram tau nrog cov tshuaj ntsuam xyuas sib haum rau hauv txoj hauj lwm thiab xim rau cov pob hauv chromatogram tau nrog cov kev daws teeb meem.

【Poob ntawm ziab】Thaum qhuav kom qhov hnyav tas li ntawm 105 degree, poob tsis ntau tshaj 10.0 ib xees ntawm nws qhov hnyav (Appendix Ⅸ G).

【Heavy hlau thiab teeb meem ntsiab】Ua raws li kev txiav txim siab ntawm cov hlau lead (Pb), Cadmium (Cd), Arsenic (As), Mercury (Hg), tooj liab (Cu) (Appendix Ⅸ B). Tsis ntau tshaj 5 ppm ntawm txhuas, tsis ntau tshaj 0.3 ppm ntawm codmiun, tsis ntau tshaj 2 ppm ntawm Arsenie, tsis ntau tshaj 0.2 ppm ntawm Mercury, tsis ntau tshaj 20 ppm ntawm Tooj.

【Ntiv tes】Ua cov txheej txheem rau kev ua haujlwm siab ua kua chromatography (AppendixVI D). Chromatographic system thiab system tsim nyog Siv octadecylsilane bonded silica gel raws li theem nyob ruaj ruaj (25cm nyob rau hauv ntev, 4.6mm nyob rau hauv txoj kab uas hla, 5µm nyob rau hauv particle inch) thiab ib tug sib tov ntawm acetonitrile thiab 2mmol / L cyclodextrin kua (24:76) raws li lub mobile theem. .As detector ib tug Sepctrophotometer teem ntawm 205 nm. Tus naj npawb ntawm theoretical

daim hlau ntawm kab ntawv tsis tsawg tshaj li 4000, suav nrog kev siv rau lub ncov ntawm asiaticoside . Kev siv tshuaj hnyav hnyav Madecassoside CRS thiab Asiaticoside CRS cais, ntxiv methanol los npaj cov kev daws teeb meem uas muaj 0.2mg ib ml, raws li cov lus qhia siv. Test solution Ua cov txheej txheem rau kev sim daws teeb meem hauv qab Assay . Cov txheej txheem Txhaj 10 µl ntawm txhua qhov kev siv cov kev daws teeb meem thiab cov tshuaj ntsuam xyuas rau hauv kab ntawv feem, thiab sau cov chromatogram.

Raws li kev soj ntsuam zoo sib xws rau chromatographic ntiv tes ntawm TCM, suav qhov zoo sib xws ntawm kev ntsuas ntsuas ntiv tes thiab siv ntiv tes, tsis tsawg dua 0.90.

Kev koom ua ke Cov xwm txheej Slope rhiab heev :1, ncov dav: 0.1, cheeb tsam tsis lees paub :20 , qhov siab tsis lees paub :0.5 .

【Assay】Ua cov txheej txheem rau kev ua haujlwm siab ua kua chromatography (Appendix VI D). Chromatographic system thiab system tsim nyog Siv octadecylsilane bonded silica gel raws li theem nyob ruaj khov thiab sib tov ntawm acetonitrile thiab 2mmol / L cyclodextrin kua

(24:76) raws li theem mobile .As detector ib sepctrophotometer teem ntawm 205 nm. Tus naj npawb ntawm cov ntawv theoretical ntawm kab ntawv tsis tsawg dua 4000, suav nrog kev siv rau lub ncov ofasiaticoside . Kev siv tshuaj hnyav hnyav Madecassoside CRS thiab Asiaticoside CRS cais, ntxiv methanol los tsim cov kev daws teeb meem uas muaj 0.2mg ib ml raws li cov kev daws teeb meem. Kev ntsuas ntsuas ntsuas qhov hnyav yog 50 mg rau 50ml ntawm ntim ntim, yaj hauv methanol, diluteto ntim, co zoo, thiab lim. Txheej txheem Txhaj 10 µl ntawm txhua qhov kev siv cov kev daws teeb meem thiab cov tshuaj ntsuam xyuas rau hauv kab ntsig, suav cov ntsiab lus. Nws muaj tsis pub tsawg tshaj 55.0 ib xees ntawm tag nrho cov ntsiab lus ntawm Madecassoside (C48H78O20) thiab Asiaticoside (C48H78O19), suav nrog siv rau lub hauv paus qhuav.

【Storage】Khaws rau hauv cov thawv kaw nruj nreem.

Qhov twg Yuav Gotu Kola Extract?

Xi'an Natural Field Bio-Technique Co., Ltd. raws li Gotu Kola Extract, uas yog tus kws tshaj lij gotu kola extract hmoov Manufacturers ntawm Herbal Extract Txij li thaum 2005. Thiab tuaj koom CPHI, Vitafoods thiab API Exhibition los ntawm 2008. Gotu Kola Extract series yog ib qho ntawm cov khoom muaj zog. Gotu Kola extract ib txwm specifications yog 10 feem pua ​​-95 feem pua ​​. Gotu kola extract hmoov yog dav siv rau kev kho mob zaub mov thiab tshuaj pleev ib ce, tshuaj. Yog tias koj xav tau cov khoom no thiab lwm yam khoom, thov hu rau peb los yog xa ib qho kev nug rau info@natural-field.comthaum twg los tau.